¿Clonar la secuencia completa de cdna del gen de la leptina bovina (usar raza)
No es necesario hacer RACE, GenBank tiene la secuencia completa:
>gi|87196504|ref|NM_173928.2| Bos taurus leptin (LEP), mRNA
CGGCGCCAGCAGCGGCGAGGCCCCAGAAGCCCATCCCGGGAAGGAAAATGCCGTGTGACCCCTGTATCG
ATTCCTGTGGCTTTGGCCCTATCTGTCTTACGTGAGCTGTGCCCATCTGCAAGGTCCAGGATGACACC
AAAACCCTCATCAAGACAATTGTCACCAGGATCAATGACATCTCACACACGCAGTCCGTCTCCTCCAAAC
AGAGGGTCACTGGTTTGGACTTCATCCCTGGGCTCCACCCTCTCCTGAGTTTGTCCAAGATGGACCAGAC
ATTGGCGATCTACCAACAGATCTCACCAGTCTGCCTTCCAGAAATGTGGTCCAAATATCCAATGACCTG
GAGAACCTCCGGGACCTTCTCCACCTGCTGGCCGCCTCCAAGAGCTGCCCCTTGCCGCAGGTCAGGGCCC
TGGAGAGCTTGGAGAGCTTGGGCGTTGTCCTGGAAGCTTCCCTCTACTCCACCGAGGTGGTGGCCCTGAG
p>CCGGCTGCAGGGTCACTACAGGACATGTTGCGGCAGCTGGACCTCAGTCCCGGGTGCTGAAGCCTTGAA
GGCCTCTCTTCCCAAAGTCCAGGGAAGAAAACCTGAGCTTCTGGCTGTCCACAGGAGAAGAGAGCCTATGT
GG GCATCCTTTTATGCAGGCCAGCGGGCCATTTCTCTCGCTCCTCAGCTGCTCTTCCAAAGGCAGAA
AACTGCGAGGCAGGAAACCAAAGATATAAATACAGATTCCACGCCCACCGGGAAGGGGGGGCCATCCAGC
AAACACTAGACCGGAGCTGGATTTTCACAGCAGTCTTCCTCCCTGTTCCAGCTCCCCTCTCACTGCATGC
TTCAGCATGACCTGGGGTGATTTCAGGCCTTTGGACCATCAAGCAAGATTCCCTCTGAGAATCCAGGGA
GCATCATGAAGGCTACAGCACATACAGCTGGATATTCCCACACAACATACGATGGAAGCATTTATTTATT
TATTATGCATTTTATTCTGAATGAATTTGAAGCAAAACACCAGCTCTTCCAGGCTCTTTGGGGTCAGCTG
GGGTGA GGAACGCTCCTGGGGTGCCCATCGACAGGCCTCACTGAGGCAAACCCATTTTGAGTGACTTGAG
GCCTTCCAAGTTTT GTTCTCCAGGGACTGGCTTTGTTTCTACTGTGACTGACTTTAAATTACAGTGTTTGC
AATGGCATTGCTCTGAATGGATCTTGAAGGACCAAGTTTGTTTTAAAAAGAAGAGATGAATTTTGTCAAG
TGTAATATATCACTGG GTATAACCCGGAGGTGGGAGATGTTTTGATGGGAGCGGGGGATCCAGAATGT
GTTTTCTGAATAACATTTGTGTGATGGACTCTTTGGATGGGGGTGAGGTCATCTTCTCATCTTTGTGGTTTT
TCATGAAGAGGATGACTCCTTCAGGGGATTGTGGGGGTTGGCCAACCATCCATGGATGGAGGTGGTGGG
GGCACTGAAGCTGAAGACCGTCGGGGTAGTGGTGAGCTCTGGCCTTCTCTGACTGTTAGAGAGTGGTCTT
GCT CATCAGGAAGTGAGGACCCCACACTGGAGATGGTGACCCCGAGCAGGGGGTCCTTGGTATGAATGG
TCTGGGTTGACCCCACACTGTGTTGATAACATGGTCGTGACCTTCTTGGGATTTGCATGCTCACCCAAA
GCAAGGCCATGTTTCCCATCCATTTGGGAAGGATTTTCATTCCAGTGGGAGAGGGAAGTATTCCAGTGTG
GACTTCAGTGGATG GTCCCTCGACCTGGGTCAGCAATGGGTCAGTTGAGGCCCAAGACCCCAGGACCAGC
CCCCAGGAGCCTCCTTCTCACTAGTGGTCATGTGCAGTAGAACAAAAGAGGAGGCTTGGGTTTCCCACCA
ACCTGCCATTGTGATGCAGCCGTCACACAACAGGAGGTGGATCTGTCCAAGGAAATTGGAATCTAAGCAA
CCAATTTTAAGACTGAGCACCTACTTGTGCTCAGCCCCAACTGGTGCTATGGGCTGAGAAGCTCACCAAA
TAAATATTAAAATGCAAGCCCTGCCCTCAGGGACCTTGCATTCCAGATGGCAGAATCCCACTCACCAGCA
TGCAAAGGCTGCCGTTTCACCATGGCAACTGAGCAGCTGAGACAGTGCAGTCCCTCAGCAGGTGGGAAATG
CTGAGCTGTGGAGGGCAGTGCCCAGGGGCCTACAGGCTAACTGTGCTTGCACTTGGCAGCAGTTTTACTT
p>TTCAGGCACGTCAGCATCTATTACTGTGATGCCACATCCCTTTGAAGCGGGATAGCTAAGAATTTAATA
AGAAGAAAATAACCTAAGACCATAACAGCAGACAGGGGGCAGGGACCAAGTCTAGAATCCTGGTCCTCTGAC
CTCCAGAGTGCCCCTTGAGCCAGGTGGTAGTCTGGAGATGTGAACAAAGTAGGGCAGGGAGGGCAGGA
GTGTTTGCTGGAAGAGAGGAGTTCCGAGGCCAATTTTGCAGGCAGTGAGGGAAGTGAATTGCCTGGAGGG
AGGAGGCTGTTTTGTTTGAAGCTTCGGTCTGAGACACCGAAGGGAGGGTGATAGGGTGAGCAGCTAGTTAC
AAAGAAAGGCAGAGGAAGCAAAAGATGGGAGGGAAGGGTTATGTTGAAGAGGACTTCAGGGGCCAAGACAT
" TAAAACAATTTTTTTTTGTTGTTCCAAAAAGTCCAAATAAATGACCTTTGCCCCTTG